Scientist l, Cell Line Development (based fully onsite)

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6 minutes ago
Type
Full time
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Related skills

cho qpcr cell line development transfection ddpcr
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📋 Description

  • Develop CHO cell lines for GMP applications in South San Francisco, CA.
  • Bench-focused role owning projects end to end with process & analytical partners.
  • Execute cell line development workflows: transfection, selection, clone screening, scale-up.
  • Characterize lines via PCR/qPCR/ddPCR and sequencing-based methods.
  • Generate monoclonal lines and maintain cell banks with proper records.
  • Design fed-batch and parallel culture studies to evaluate productivity and stability.

🎯 Requirements

  • Bachelor’s degree (5+ yrs) or Master’s (2+ yrs) in biologics or related field.
  • Hands-on aseptic mammalian cell culture with CHO focus.
  • Proficiency in DNA/RNA extraction and PCR-based analyses (qPCR/ddPCR).
  • Experience creating stable CHO lines and using transposase/recombinase systems.
  • Cryopreservation of CHO cells and cell bank management.
  • Experience with cloning, screening in 96-well/24-deep-well plates, and data interpretation.

🎁 Benefits

  • AbbVie-wide benefits and compensation program.
  • Opportunity to work on diverse platform/non-platform projects.
  • Equal opportunity employer.
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